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Adjuvants such as aluminum salts are used as additives in vaccines to enhance immune responses.

A) True
B) False

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Which of the following are sources of antibodies for serological testing?


A) cells producing monoclonal antibodies
B) vaccinated animals
C) vaccinated animals, cells producing monoclonal antibodies, and viral cultures
D) vaccinated animals and cells producing monoclonal antibodies
E) viral cultures

F) All of the above
G) A) and D)

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A vaccine against HIV proteins made by a genetically -engineered vaccinia virus that has infected a eukaryotic cell line is a(n)


A) toxoid vaccine.
B) subunit vaccine.
C) conjugated vaccine.
D) nucleic acid vaccine.
E) inactivated whole-agent vaccine.

F) A) and B)
G) A) and C)

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Which of the following statements about measles is FALSE?


A) It is preventable by vaccination.
B) Annually, it kills thousands of children worldwide.
C) It is a serious disease.
D) The disease has been eradicated in the United States.
E) Complications include pneumonia, encephalitis, and death.

F) C) and D)
G) B) and E)

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The following steps are used to produce monoclonal antibodies. What is the fourth step?


A) Culture the hybridoma in a selective medium.
B) A B cell is activated to produce antibodies.
C) Isolate antibody-producing B cells.
D) Vaccinate a mouse.
E) Fuse a B cell to a myeloma cell.

F) A) and B)
G) A) and C)

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Figure 18.1 is an illustration of a(an)


A) negative indirect ELISA test.
B) positive indirect ELISA test.
C) complement fixation test.
D) precipitation test.
E) hemagglutination test.

F) B) and D)
G) C) and D)

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Patientʹs serum, influenza virus, and red blood cells are mixed in a tube. What happens if the patient has antibodies against influenza virus?


A) hemolysis
B) agglutination
C) hemagglutination
D) complement fixation
E) hemagglutination-inhibition

F) C) and D)
G) A) and B)

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Agglutination tests use particulate antigens while precipitation tests use soluble antigens.

A) True
B) False

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Which of the following uses fluorescent-labeled antibodies?


A) neutralization
B) complement fixation
C) precipitation
D) flow cytometry
E) agglutination

F) C) and D)
G) A) and B)

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In an agglutination test, eight serial dilutions to determine antibody titer were set up. Tube #1 contained a 1:2 dilution; tube #2, a 1:4, etc. If tube #6 is the last tube showing agglutination, what is the antibody titer?


A) 6
B) 1:6
C) 1:32
D) 32
E) 64

F) D) and E)
G) C) and E)

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In a direct ELISA test to screen for drugs in a patientʹs urine, what is the third step in the test process?


A) enzyme-labeled antibodies against the drug being tested is added
B) substrate for the enzyme is added
C) antibody against the drug being tested is added
D) the patientʹs urine sample is diluted

E) C) and D)
F) A) and B)

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Which of the following tests is MOST useful in determining the presence of AIDS antibodies?


A) neutralization
B) agglutination
C) complement fixation
D) indirect ELISA
E) direct fluorescent-antibody

F) A) and C)
G) All of the above

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Describe an ELISA test to detect the presence of HIV antibodies in a patient.

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ELISA, or enzyme-linked immunosorbent as...

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Which of the following is a test to determine the presence of soluble antigens in a patientʹs saliva?


A) immunofluorescence
B) precipitation reaction
C) passive agglutination reaction
D) neutralization reaction
E) direct agglutination reaction

F) B) and C)
G) B) and E)

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Isolated and purified hepatitis B virus surface antigen can be used in a(n)


A) inactivated whole-agent vaccine.
B) attenuated whole-agent vaccine.
C) conjugated vaccine.
D) subunit vaccine.
E) toxoid vaccine.

F) A) and B)
G) All of the above

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Table 18.1 Antibody Titer  Day 1  Day 7 Day 14  Day 21  Patient A 00256512 Patient B 1282565121024 Patient C 0000 Patient D 128128128128\begin{array} { | l | l | l | l | l | } \hline & \text { Day 1 } & \text { Day } 7 & \text { Day 14 } & \text { Day 21 } \\\hline \text { Patient A } & 0 & 0 & 256 & 512 \\\hline \text { Patient B } & 128 & 256 & 512 & 1024 \\\hline \text { Patient C } & 0 & 0 & 0 & 0 \\\hline \text { Patient D } & 128 & 128 & 128 & 128 \\\hline\end{array} -In Table 18.1, who showed seroconversion during these observations?


A) Patient A
B) Patient B
C) Patient C
D) Patient D

E) B) and C)
F) A) and D)

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In your work in a county public health clinic, you encounter parents of a three -month-old who are considering not vaccinating their child. What concerns might the parents raise regarding vaccine safety? As a microbiology student and public health care worker, what do you say to these parents regarding the risks of not vaccinating their child?

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The concerns that parents might raise re...

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The clumping of test red blood cells indicates a negative test result (no antibodies against the virus in the patientʹs serum) in the


A) precipitation test.
B) indirect agglutination test.
C) direct agglutination test.
D) viral hemagglutination inhibition test.
E) complement-fixation test.

F) None of the above
G) D) and E)

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