Question 1

What occurs during the annealing stage of a PCR reaction?

Accepted Answer

A)   The DNA strands separate
B)   The DNA polymerase copies the template DNA
C)   The primers bind to the ends of the template DNA
D)   The reaction stops
E)   All of the answers are correctF) All of the above
G) A) and B)

Question 2

Which of the following is used to identify a specific protein in a library?

Accepted Answer

A)   Western blotting
B)   Northern blotting
C)   Southern blotting
D)   Eastern blottingE) A) and B)
F) A) and D)

Question 3

Which of the following techniques may be used to determine DNA-protein interactions?

Accepted Answer

A)   Western blotting
B)   PCR
C)   DNAse I foot printing
D)   Restriction enzyme analysisE) B) and D)
F) All of the above

Question 4

Small circular pieces of bacterial DNA that are used as vectors in cloning experiments are called .

Accepted Answer

A)   Phages
B)   Viruses
C)   Plasmids
D)   Chromosomes
E)   None of the answers are correctF) A) and D)
G) A) and C)

Question 5

conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.

Accepted Answer

A)   High stringency
B)   Low stringencyC) A) and B)
D) undefined

Question 6

The DNA Pol III enzyme can use RNA to make a DNA strand.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following would contain both vector DNA and chromosomal DNA?

Accepted Answer

A)   Recircularized vector
B)   Hybrid vector
C)   Both vectors
D)   Neither vectorE) All of the above
F) A) and C)

Question 8

Which of the following is correct regarding the first cloning experiment?

Accepted Answer

A)   It utilized the EcoR1 restriction enzyme
B)   The vector was a plasmid called pSC101
C)   The vector had a selectable marker for tetracycline resistance
D)   The process involved the cloning of kanamycinR gene
E)   All of the answers are correctF) A) and D)
G) B) and C)

Question 9

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Accepted Answer

A) True 
 B)False

Question 10

The presence of a specific plasmid in a bacterial cell may be determined if the plasmid .

Accepted Answer

A)   Is exceptionally large
B)   Is an expression vector
C)   Contains pieces of a viral genome
D)   Contains a selectable marker
E)   None of the answers are correctF) All of the above
G) B) and D)

Question 11

What is the purpose of RNaseH in the making of cDNA?

Accepted Answer

A)   It copies the RNA into a complementary DNA strand
B)   It reforms the sugar-phosphate backbone
C)   It generates short RNAs that are used as primers
D)   It proofreads the cDNA for errorsE) All of the above
F) C) and D) C

Question 12

DNA ligase is needed in a cloning experiment for what reason?

Accepted Answer

A)   To proofread the vector for mistakes
B)   To reestablish the stable sugar-phosphate backbone of the DNA molecule
C)   To allow the vector to enter into the cell
D)   As a selectable markerE) C) and D)
F) B) and C)

Question 13

Which of the following is NOT a stage of a PCR reaction?

Accepted Answer

A)   DNA is heated to a temperature at which the hydrogen bonds holding the strands break.
B)   The primers bind to the complementary sequences on the template DNA.
C)   DNA gyrase enzyme relieves the supercoiling ahead of the replication fork.
D)   The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA.E) B) and C)
F) A) and D) C

Question 14

Which of the following is used to identify a specific RNA in a library?

Accepted Answer

A)   Western blotting
B)   Northern blotting
C)   Southern blotting
D)   Eastern blottingE) B) and C)
F) A) and B) B

Question 15

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Accepted Answer

A)   Sequencing ladders
B)   Automated sequencing
C)   Base-specific DNA cleavage
D)   All of the answers are correctE) None of the above
F) C) and D)

Question 16

Dideoxy nucleotides are used in which of the following techniques?

Accepted Answer

A)   RT-PCR
B)   DNA sequencing
C)   DNAse I foot printing
D)   Restriction mapping
E)   Gene cloningF) A) and D)
G) A) and B)

Question 17

A cDNA library differs from a DNA library in which way?

Accepted Answer

A)   It consists solely of RNA molecules
B)   It contains many copies of the gene of interest
C)   It contains only coding sequences, not introns
D)   It is typically 10-100 times the size of a DNA libraryE) None of the above
F) B) and C)

Question 18

How might a probe for a colony hybridization experiment be obtained?

Accepted Answer

A)   By using a known copy of the gene
B)   By using a similar gene from a closely related species
C)   Using antibodies that detect the clones expressing the protein
D)   By using small fragments of the protein to generate probes
E)   All of the answers are possibleF) C) and E)
G) B) and E)

Question 19

Which of the following is needed for a PCR reaction?

Accepted Answer

A)   Template DNA
B)   Primers
C)   DNA polymerase
D)   Deoxynucleotide triphosphates
E)   All of the answers are correctF) A) and E)
G) B) and E)

Question 20

Antibodies can be used as a probe for which of the following techniques?

Accepted Answer

A)   Western blotting
B)   Northern blotting
C)   Southern blotting
D)   Eastern blottingE) C) and D)
F) A) and D)

Exam 18: Recombinant Dna Technology

What occurs during the annealing stage of a PCR reaction?

Correct AnswerverifiedShow Answer

Which of the following is used to identify a specific protein in a library?

Correct AnswerverifiedShow Answer

Which of the following techniques may be used to determine DNA-protein interactions?

Correct AnswerverifiedShow Answer

Small circular pieces of bacterial DNA that are used as vectors in cloning experiments are called .

Correct AnswerverifiedShow Answer

conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.

Correct AnswerverifiedShow Answer

The DNA Pol III enzyme can use RNA to make a DNA strand.

Correct AnswerverifiedShow Answer

Which of the following would contain both vector DNA and chromosomal DNA?

Correct AnswerverifiedShow Answer

Which of the following is correct regarding the first cloning experiment?

Correct AnswerverifiedShow Answer

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Correct AnswerverifiedShow Answer

The presence of a specific plasmid in a bacterial cell may be determined if the plasmid .

Correct AnswerverifiedShow Answer

What is the purpose of RNaseH in the making of cDNA?

Correct AnswerverifiedC

DNA ligase is needed in a cloning experiment for what reason?

Correct AnswerverifiedShow Answer

Which of the following is NOT a stage of a PCR reaction?

Correct AnswerverifiedC

Which of the following is used to identify a specific RNA in a library?

Correct AnswerverifiedB

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Correct AnswerverifiedShow Answer

Dideoxy nucleotides are used in which of the following techniques?

Correct AnswerverifiedShow Answer

A cDNA library differs from a DNA library in which way?

Correct AnswerverifiedShow Answer

How might a probe for a colony hybridization experiment be obtained?

Correct AnswerverifiedShow Answer

Which of the following is needed for a PCR reaction?

Correct AnswerverifiedShow Answer

Antibodies can be used as a probe for which of the following techniques?

Correct AnswerverifiedShow Answer

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C

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C

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B

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