Question 1

Antibodies can be used as a probe for which of the following techniques?

Accepted Answer

A)   Western blotting
B)   Northern blotting
C)   Southern blotting
D)   Eastern blottingE) A) and D)
F) None of the above

Question 2

Hematopoietic stem cells are ________.

Accepted Answer

A)   pluripotent
B)   totipotent
C)   unipotent
D)   multipotentE) B) and D)
F) C) and D) D

Question 3

The origins of which of the following cell types creates the least amount of ethical debate?

Accepted Answer

A)   embryonic germ cells
B)   hematopoietic stem cells
C)   embryonic stem cells
D)   All of the answers are equal.E) C) and D)
F) A) and B)

Question 4

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Accepted Answer

A) True 
 B)False  True

Question 5

Which of the following individual(s) developed the process of the polymerase chain reaction?

Accepted Answer

A)   Watson and Crick
B)   McClintock
C)   Mullis
D)   PaulingE) B) and C)
F) A) and D)

Question 6

Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.

Accepted Answer

A) True 
 B)False  False

Question 7

Which of the following cells is not pluripotent?

Accepted Answer

A)   embryonic stem cells
B)   embryonic germ cells
C)   both are pluripotent
D)   neither are pluripotentE) A) and D)
F) C) and D)

Question 8

What is a DNA library?

Accepted Answer

A)   a collection of vectors, each containing a fragment of the genome or DNA of interest
B)   a complete sequence of the genetic material in an organism
C)   an electronic file that may be used for additional genetic analysis
D)   a collection of mRNA from a given cellE) B) and C)
F) All of the above

Question 9

Since cDNA is made from RNA,it lacks introns and contains only the coding sequence for the functional protein.

Accepted Answer

A) True 
 B)False

Question 10

In a cloning experiment with chromosomal DNA,you use a vector that contains a lacZ gene as a selectable marker.If the competent cells are grown on X-Gal and IPTG,which colonies would contain cloned chromosomal DNA?

Accepted Answer

A)   the white colonies
B)   the blue colonies
C)   half the total colonies
D)   none of the coloniesE) B) and C)
F) A) and D)

Question 11

The primary interest of genetic research into stem cells is the desire to clone a human being.

Accepted Answer

A) True 
 B)False

Question 12

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Accepted Answer

A)   automated DNA sequencing
B)   base-specific DNA cleavageC) A) and B)
D) undefined

Question 13

DNA ligase is needed in a cloning experiment for what reason?

Accepted Answer

A)   to proofread the vector for mistakes
B)   to reestablish the stable sugar-phosphate backbone of the DNA molecule
C)   to allow the vector to enter into the cell
D)   as a selectable markerE) A) and B)
F) A) and C)

Question 14

A plasmid that contains separate origins of replication for two different species is called a(n) ________.

Accepted Answer

A)   R factor
B)   expression vector
C)   viral vector
D)   shuttle vectorE) A) and B)
F) None of the above

Question 15

What is the origin of restriction endonucleases?

Accepted Answer

A)   They are part of DNA repair mechanisms in eukaryotic cells.
B)   They are a defense mechanism against viruses in bacteria.
C)   They are replication enzymes of yeast.
D)   They are transposable elements of Drosophila.E) A) and D)
F) A) and B)

Question 16

Select items that describe a stage of a PCR reaction.Choose all that apply.

Accepted Answer

A)   DNA is heated to a temperature that causes the strands to separate.
B)   Primers bind to complementary sequences on the template DNA.
C)   DNA gyrase enzyme relieves the supercoiling ahead of the replication fork.
D)   The DNA polymerase synthesizes a new strand of DNA that is complementary to the template DNA.E) B) and C)
F) A) and B)

Question 17

The first organism ever cloned by researchers was Dolly the sheep in 1997.

Accepted Answer

A) True 
 B)False

Question 18

The isolation and copying of a gene,usually in large quantities is called gene cloning.

Accepted Answer

A) True 
 B)False

Question 19

What is a property of stem cells?

Accepted Answer

A)   They can differentiate into one or more specialized cell types.
B)   They can form any tissue in the body.
C)   They are only capable of one to two cell divisions.
D)   They will only form hematopoietic cells.E) None of the above
F) All of the above

Question 20

Which procedure is used to identify a specific protein within a mixture of many different protein molecules?

Accepted Answer

A)   Western blotting
B)   Northern blotting
C)   Southern blotting
D)   Eastern blottingE) None of the above
F) A) and C)

Exam 20: Molecular Technologies

Antibodies can be used as a probe for which of the following techniques?

Correct AnswerverifiedShow Answer

Hematopoietic stem cells are ________.

Correct AnswerverifiedD

The origins of which of the following cell types creates the least amount of ethical debate?

Correct AnswerverifiedShow Answer

A vector is a small segment of DNA that a gene of interest is inserted into for cloning.

Correct AnswerverifiedTrue

Which of the following individual(s) developed the process of the polymerase chain reaction?

Correct AnswerverifiedShow Answer

Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.

Correct AnswerverifiedFalse

Which of the following cells is not pluripotent?

Correct AnswerverifiedShow Answer

What is a DNA library?

Correct AnswerverifiedShow Answer

Since cDNA is made from RNA,it lacks introns and contains only the coding sequence for the functional protein.

Correct AnswerverifiedShow Answer

In a cloning experiment with chromosomal DNA,you use a vector that contains a lacZ gene as a selectable marker.If the competent cells are grown on X-Gal and IPTG,which colonies would contain cloned chromosomal DNA?

Correct AnswerverifiedShow Answer

The primary interest of genetic research into stem cells is the desire to clone a human being.

Correct AnswerverifiedShow Answer

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Correct AnswerverifiedShow Answer

DNA ligase is needed in a cloning experiment for what reason?

Correct AnswerverifiedShow Answer

A plasmid that contains separate origins of replication for two different species is called a(n) ________.

Correct AnswerverifiedShow Answer

What is the origin of restriction endonucleases?

Correct AnswerverifiedShow Answer

Select items that describe a stage of a PCR reaction.Choose all that apply.

Correct AnswerverifiedShow Answer

The first organism ever cloned by researchers was Dolly the sheep in 1997.

Correct AnswerverifiedShow Answer

The isolation and copying of a gene,usually in large quantities is called gene cloning.

Correct AnswerverifiedShow Answer

What is a property of stem cells?

Correct AnswerverifiedShow Answer

Which procedure is used to identify a specific protein within a mixture of many different protein molecules?

Correct AnswerverifiedShow Answer

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False

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